Fig 1: The mRNA and protein expression of NOL4 in normal tissues and small-cell lung cancer (SCLC) cell lines. RT-PCR was performed on mRNA expression of NOL4 in normal tissues (A) and SCLC lines (B). Western blot analysis was performed to evaluate the protein expression of NOL4 in SCLC cell lines, and the intensity ratio of each band was analyzed by Image J (C). Normal tissues were indicated as follows: 1, spleen; 2, thymus; 3, prostate; 4, ovary; 5, small intestine; 6, colon; 7, leukocyte; 8, heart; 9, brain; 10, placenta; 11, lung; 12, pancreas; 13, liver; 14, skeletal muscle; 15, kidney; and 16, testis. The cDNA templates were normalized using GAPDH, as shown in the bottom panel.
Fig 2: The mRNA expression of NOL4 in several cancer cell lines.
Fig 3: Immunohistochemical staining of NOL4 proteins. Panels refer to representative sections showing positive staining (A) and negative staining (B). Original magnification is 100×.
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